Reconstituted system reference state
The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.
SEEK ID: https://sandbox11.fairdomhub.org/assays/1
Experimental assay
Projects: Default Project
Investigation: Central Carbon Metabolism of Sulfolobus solfataricus
Study: Carbon loss at high T
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: Sulfolobus solfataricus
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Creators and SubmitterViews: 12
Created: 21st Sep 2026 at 14:38
Last updated: 21st Sep 2026 at 14:39
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Projects: Default Project
Institutions: Default Institution
This is a test programme for the SEEK sandbox.
Projects: Default Project
Web page: http://www.seek4science.org
A description for the default project
Programme: Default Programme
Public web page: https://www.seek4science.org
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Submitter: Guest User
Studies: Carbon loss at high T
Assays: Assay stream, Model reconstituted system, Reconstituted system reference state
The carbon loss at high T description will be here but I am currently not imaginative enough.
Submitter: Guest User
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Assays: Assay stream, Model reconstituted system, Reconstituted system reference state
Experimental data for the reconstituted system are plotted together with the model prediction.
The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed.
Standard operating procedure for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus to study metabolic pathway efficiency at high temperatures.
Creator: Guest User
Submitter: Guest User
Abstract (Expand)
Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, J. Snoep
Date Published: 21st Sep 2026
Publication Type: Journal Article
