Default Project

This is a test programme for the SEEK sandbox.

An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.

The carbon loss at high T description will be here but I am currently not imaginative enough.

A large scale bioreactor with a 1000 mL reservoir.

The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.

Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.

A stream of assays? This is a test assay stream for the example data.

Paired-end whole genome sequencing reads (R1/R2 FASTQ, gzip) uploaded during the SEEK API workshop.

Experimental data for the reconstituted system are plotted together with the model prediction.

The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed.

The PGK, GAPDH, TPI and FBPAase were modelled together using the individual rate equations. Closed system.

Standard operating procedure for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus to study metabolic pathway efficiency at high temperatures.

Four enzymes of the gluconeogenic pathway in Sulfolobus solfataricus were purified and kinetically characterized. The enzymes were reconstituted in vitro to quantify the contribution of temperature instability of the pathway intermediates to carbon loss from the system. The reconstituted system, consisting of phosphoglycerate kinase, glyceraldehyde 3-phosphate dehydrogenase, triose phosphate isomerase and the fructose 1,6-bisphosphate aldolase/phosphatase, maintained a constant consumption rate ...

Event for publication

This document describes the experimental setup and procedures used for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus.

Sample type for purified enzyme preparations used in reconstituted systems

Sample type for bacterial culture experiments related to thermophile studies

A collection of data files, models, SOPs and publications related to the reconstituted gluconeogenic enzyme system from Sulfolobus solfataricus.