Carbon loss at high T
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The carbon loss at high T description will be here but I am currently not imaginative enough.
SEEK ID: https://sandbox11.fairdomhub.org/studies/1
Central Carbon Metabolism of Sulfolobus solfataricus
Projects: Default Project
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Creators and SubmitterViews: 11
Created: 21st Sep 2026 at 14:38
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Projects: Default Project
Institutions: Default Institution
This is a test programme for the SEEK sandbox.
Projects: Default Project
Web page: http://www.seek4science.org
A description for the default project
Programme: Default Programme
Public web page: https://www.seek4science.org
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Submitter: Guest User
Studies: Carbon loss at high T
Assays: Assay stream, Model reconstituted system, Reconstituted system reference state
A large scale bioreactor with a 1000 mL reservoir.
The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.
Submitter: Guest User
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.
Submitter: Guest User
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
A stream of assays? This is a test assay stream for the example data.
Submitter: Guest User
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Experimental data for the reconstituted system are plotted together with the model prediction.
Creators: Admin User, Person A, Person B
Submitter: Guest User
The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed.
Creator: Guest User
Submitter: Guest User
The PGK, GAPDH, TPI and FBPAase were modelled together using the individual rate equations. Closed system.
Creator: Guest User
Submitter: Guest User
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
Standard operating procedure for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus to study metabolic pathway efficiency at high temperatures.
Creator: Guest User
Submitter: Guest User
Abstract (Expand)
Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, J. Snoep
Date Published: 21st Sep 2026
Publication Type: Journal Article
Sample type for purified enzyme preparations used in reconstituted systems
Enzyme Name (String) *, EC Number (String) , Concentration (Real number) ( mg/ml ) *, Specific Activity (Real number) , Storage Temperature (Integer) ( °C ) , Purification Steps (Integer)
Sample type for bacterial culture experiments related to thermophile studies
Culture Name (String) *, Strain Used (String) *, Growth Temperature (Real number) ( °C ) *, Culture Volume (Real number) ( mL ) , pH (Real number) , Growth Phase Complete (Boolean)
