Guest User
SEEK ID: https://sandbox11.fairdomhub.org/people/2
Location:
United Kingdom
ORCID: Not specified
Joined: 21st Sep 2026
Expertise: Not specified
Tools: Not specified
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Their tagsRelated items
This is a test programme for the SEEK sandbox.
Projects: Default Project
Web page: http://www.seek4science.org
A description for the default project
Programme: Default Programme
Public web page: https://www.seek4science.org
ROR ID: Not specified
Department: Not specified
Country: United Kingdom
City: Manchester
Web page: Not specified
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Submitter: Guest User
Studies: Carbon loss at high T
Assays: Assay stream, Model reconstituted system, Reconstituted system reference state
The carbon loss at high T description will be here but I am currently not imaginative enough.
Submitter: Guest User
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Assays: Assay stream, Model reconstituted system, Reconstituted system reference state
A large scale bioreactor with a 1000 mL reservoir.
The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.
Submitter: Guest User
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.
Submitter: Guest User
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
A stream of assays? This is a test assay stream for the example data.
Submitter: Guest User
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Experimental data for the reconstituted system are plotted together with the model prediction.
Creators: Admin User, Person A, Person B
Submitter: Guest User
The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed.
Creator: Guest User
Submitter: Guest User
The PGK, GAPDH, TPI and FBPAase were modelled together using the individual rate equations. Closed system.
Creator: Guest User
Submitter: Guest User
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
Standard operating procedure for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus to study metabolic pathway efficiency at high temperatures.
Creator: Guest User
Submitter: Guest User
Abstract (Expand)
Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, J. Snoep
Date Published: 21st Sep 2026
Publication Type: Journal Article
Four enzymes of the gluconeogenic pathway in Sulfolobus solfataricus were purified and kinetically characterized. The enzymes were reconstituted in vitro to quantify the contribution of temperature instability of the pathway intermediates to carbon loss from the system. The reconstituted system, consisting of phosphoglycerate kinase, glyceraldehyde 3-phosphate dehydrogenase, triose phosphate isomerase and the fructose 1,6-bisphosphate aldolase/phosphatase, maintained a constant consumption rate ...
Creator: Guest User
Submitter: Guest User
Event for publication
Start Date: 21st Sep 2026
End Date: 22nd Sep 2026
Event type: Not specified
This document describes the experimental setup and procedures used for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus.
Creator: Admin User
Submitter: Guest User
Sample type for purified enzyme preparations used in reconstituted systems
Enzyme Name (String) *, EC Number (String) , Concentration (Real number) ( mg/ml ) *, Specific Activity (Real number) , Storage Temperature (Integer) ( °C ) , Purification Steps (Integer)
Sample type for bacterial culture experiments related to thermophile studies
Culture Name (String) *, Strain Used (String) *, Growth Temperature (Real number) ( °C ) *, Culture Volume (Real number) ( mL ) , pH (Real number) , Growth Phase Complete (Boolean)
A collection of data files, models, SOPs and publications related to the reconstituted gluconeogenic enzyme system from Sulfolobus solfataricus.
